American Society for Clinical Pathology (ASCP) Histotechnologist Practice Test

Question: 1 / 400

Which factor most significantly affects the rate of tissue fixation during embedding?

The temperature of the fixative

The volume ratio of fixative to tissue

The volume ratio of fixative to tissue plays a crucial role in the rate of tissue fixation during embedding because it directly influences the penetration and diffusion of the fixative into the tissue. An adequate ratio ensures that there is sufficient fixative available to interact with the tissue effectively, allowing it to permeate fully and rapidly. If the tissue is too large relative to the volume of the fixative, it may not be fixed thoroughly, which can lead to poor quality samples and inaccurate results in later histological analysis.

Additionally, higher ratios typically enhance the fixation process because they provide the fixative with more opportunities to interact with the tissue structures, leading to more effective preservation of cellular morphology and proteins. Therefore, maintaining an appropriate volume ratio is essential for optimal fixation and the integrity of the tissue during subsequent handling and embedding.

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The age of the tissue sample

The type of embedding medium used

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